Thank you for your interest in our work. Our policy is to make available all published reagents created in our lab. Most of our most frequently requested materials can be obtained from the external sources listed below. We receive so many requests that we ask you to PLEASE use these inexpensive sources if you can. Our alpha-synuclein related strains are only available for non-commercial purposes. The yeast expression library is available from the HIP laboratory as described below.

For other materials not available from public resources, or if you can't afford the costs, please contact Sue's Administrative Coordinator, lindquist_admin@wi.mit.edu, phone: 617-258-5184, fax: 617-258-7226. This is also the best way to contact Sue or other lab members for additional information or for requests for collaborations.

Unfortunately, we CANNOT provide reagents used in our publications that are created by other laboratories.

Our plasmids have been deposited in Addgene's collection (click button below to browse):

Find Lindquist Lab Plasmids

Addgene is a non-profit plasmid repository, funded by processing fees paid by the requesting scientists. It costs $65 per plasmid to request a plasmid from Addgene. Significant discounts may be made available to laboratories in need that cannot afford the processing fees. Please contact the Addgene representative if you wish to discuss a discount.

For vectors described in: "A suite of Gateway™ vectors for high-throughput genetic analysis in Saccharomyces cerevisiae". Simon Alberti, Aaron D. Gitler, and Susan Lindquist." 2007. Yeast. 24(10):913-919. [PDF 972 KB]

pAG Vectors is a collection of 288 yeast Gateway expression vectors. These "Advanced Gateway" vectors are modifications of the pRS series plasmids and allow for inducible or constitutive expression, as well as fusion to a number of protein tags and fluorescent proteins. We have also made all of these vectors available through Addgene.

 

Yeast Gal ORF expression library

The expression library used for the genetic screens described in Cooper, Gitler et al. (2006) is available from HIP, the Harvard Institute of Proteomics.

FLEXgene Library, plasmidhelp@hms.harvard.edu

Procedure for Obtaining Alpha-synuclein Reagents

Download MTAs:

1. To request materials described in "Yeast Cells Provide Insight into Alpha-Synuclein Biology and Pathobiology" by Tiago Outeiro and Susan Lindquist, click here for Material Transfer Agreement.

2. To request materials described in "Alpha-Synuclein Blocks ER-Golgi Traffic and Rab1 Rescues Neuron Loss in Parkinson's Models" by Antony A. Cooper, Aaron D. Gitler, Anil Cashikar, Cole M. Haynes, Kathryn J. Hill, Bhupinder Bhullar, Kangning Liu, Kexiang Xu, Katherine E. Strathearn, Fang Liu, Songsong Cao, Kim A. Caldwell, Guy A. Caldwell, Gerald Marsischky, Richard D. Kolodner, Joshua LaBaer, Jean-Christophe Rochet, Nancy M. Bonini, and Susan Lindquist, click here for Material Transfer Agreement.

Alternative sources for other specific reagents:

1. Hsp70 monoclonal antibody 7.10, published in 1983 VELAZQUEZ et al. J. Cell Biol. 96:286-290.
   This is available commercially from Affinity Bioreagents, order #MA3-001.


2. Hsp104 polyclonal antibody, generated to the C-terminus of Hsp104, published in Sanchez, Y. and Lindquist, S.L. (1990) Science 248: 1112-1115.
   This is available commercially from Stressgen Bioreagents, catalog # SPA-1040D.


3. Hsp70 monoclonal antibody 7FB, generated to Drosophila Hsp70, described in Velazquez, J.M. and Lindquist, S.L. (1984) Cell 36:655-662.
   This is a very old antibody and our supply is almost exhausted. Antibodies that recognize Drosophila Hsp70 are available from Stressgen Bioreagents.


4. We no longer maintain Drosophila stocks.
   Some of them can be obtained from the Bloomington stock center. FLP stocks can be obtained from Kent Golic, golic at biology.utah.edu.


5. Sup35 peptide antibodies, as described in Patino, MM, et al. (1996) Science 273: 622-626 are no longer being sent out. Our supplies are almost exhausted.


6. Hsp82 / Hsc82 peptide antibodies, as described in Borkovich KA, et al. (1989) Molecular and Cellular Biology 9:3919-3939 are no longer being sent out. Our supplies are almost exhausted.

Online Request Form (for all other reagents)

We need to know the organism you are working with, the specific name for the material you want, and the reference to the paper in which the material was published. We have many thousands of strains and plasmids and hundreds of antibodies. A request for "an hsp70 plasmid," "an hsp90 antibody," "an Hsp82 mutant" is NOT sufficient. It is essential to fill the form out completely.