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We are distributing many commonly-requested reagents through the non-profit
ADDGENE organization.
These reagents can be ordered at the following link: Sabatini
Lab Reagents Distributed by ADDGENE.
Sabatini Lab Reagents Available from Addgene:
mTOR-related
rat
mTOR sequence in GenBank
human mTOR siRNAs
myc-mTOR wild-type
(rat) , prk5 vector
myc-mTOR
kinase-dead (rat), prk5 vector
mTOR_1 shRNA (human), pLKO vector
mTOR_2 shRNA (human),
pLKO vector
raptor-related
human
raptor sequence in GenBank human
raptor siRNAs
myc-raptor wild-type (human),
prk5 vector
HA-raptor wild-type (human), prk5 vector
TAP-raptor wild-type (human), pMSCV vector
myc-raptor
mutants 1,2,3,4,7 and 9 (human), prk5 vector
raptor_1 shRNA
(human),
pLKO vector
raptor_2 shRNA (human),
pLKO vector
rictor-related
human
rictor sequence in GenBank human rictor
siRNAs
myc-rictor
wild-type (human), prk5 vector
myc-rictor
wild-type (corrected)(human), prk5 vector
[mutations
introduced
during subcloning are corrected in this version]
rictor_1
shRNA (human),
pLKO vector
rictor_2 shRNA (human),
pLKO vector
GbL/mLST8-related
human
mLST8 sequence in GenBank human
GbL siRNAs
HA-GbL/mLST8
wild-type (human), prk5 vector
HA-GbL/mLST8
mutants 1,2,5,6, and 7 (human), prk5 vector
TAP-mLST8 wild-type (human), pMSCV vector
mSin1-related
human
mSin1 sequence in GenBank
mSin1.1-myc
(human), pMSCV vector
mSin1.2-myc
(human), pMSCV vector
mSin1.4-myc
(human), pMSCV vector
mSin1.5-myc
(human), pMSCV vector
Other
reagents at Addgene
Please
examine the Addgene website for many other reagents (S6K1-related, empty
vectors, plasmids encoding control proteins)
Other
Reagents
For reagents not distributed by ADDGENE, we
are happy to fulfill reasonable requests for published reagents to qualified
researchers. Commercial entities should contact the Intellectual Property
office (617-258-5853) for information on how to obtain reagents from the
Whitehead Institute. To obtain published reagents from the lab not available
through Addgene, academic researchers must send us a completed Implementation
Letter for the Uniform Biological Material Transfer Agreement (UBMTA).
Below are the reagents available as well as the reagent names you should
use in your request and the instructions for filling out the UBMTA implementation
letter. For reagents not listed please first contact the lab by email
to see if it is available.
Follow the steps below to obtain these reagents from the lab
- Download the UBMTA Implementation Letter: Click HERE to download an MS Word file containing the letter. When you open the file you will see sections indicated that you need to fill out.
- Using a word processor fill out the UBMTA Implementation Letter, print it, sign it and have it signed by an appropriate official of your institution. To complete the letter you will need: a list of the materials/reagents you are requesting (please limit yourself to no more than 4 reagents and use the names indicated below) and a brief description describing the experiments you wish to undertake with the reagents. When you return the completed letter you will also need to send us a shipping address and telephone number, and, importantly, a FedEx account number we can use to cover the shipping charges.
- After completing the UBMTA Implementation Letter mail or fax it to the attention of Lahra Tillman, Sabatini Lab, Whitehead Institute, Nine Cambridge Center, Cambridge, MA 02142-1479. Fax #: 617-452-3566. Reagents are usually sent out in a week or two after a request is received.
- When you return the completed and signed UBMTA Implementation Letter please remember to send us a shipping address and telephone number, and a FedEx account number we can use to cover the shipping charges. Good luck with your experiments.
