We are creating a viral-vector-based RNA interference (RNAi) library
against mouse and human genes. Short hairpin RNAs (shRNAs) that generate
siRNAs intracellularly are expressed from lentivirus and Moloney leukemia
virus vectors, allowing screening in a wide range of mammalian cell
types. The lentiviral vector permits infection of differentiated and
non-dividing cells, such as neurons and dendritic cells.
The libraries will consist of bacterial glycerol stocks harboring sequence-verified
shRNA Moloney and lentiviral vectors for mouse and human genes with
4-5 monoclonal stocks per gene. These libraries will be statistically
or comprehensively validated for knockdown efficiency in mammalian cells.
Methods for efficient screening with this library are being developed
and will be shared. Our intent is that these libraries will be freely
distributed to academic scientists and we plan on making subsets of
the libraries available as they are produced.
The creation of the RNAi library is the joint effort of several research
groups at the Whitehead Institute, the Broad Institute, the Dana Farber
Cancer Institute, and the Massachusetts General Hospital.
Consortium Scientists
David Root, Project Leader, Broad Institute
Greg Hinkle, Dana Farber, Whitehead Institute,
Biao Luo, Broad Institute
Chad Nusbaum, Broad Institute
Xiaoping Yang, Broad Institute
Founding Laboratories
Nir Hacohen, Massachusetts General Hospital
William Hahn, Dana Farber Cancer Institute
Eric Lander, Broad Institute
David Sabatini, Whitehead Institute, MIT
Sheila Stewart, Washington University
Brent Stockwell, Columbia University
Associated Laboratories
David Bartel, Whitehead Institute, MIT
Todd Golub, Broad Institute, Dana Farber
Harvey Lodish, Whitehead Institute, MIT
Stuart Schreiber, Broad Institute, Harvard
