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Sive Lab Protocols General Lab Protocols | Microdissection | Microinjection | Microscopy | Molecular Biology Protocols | Mutagenesis | Staining

 

 

 

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General Lab Protocols

Recipes

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Microdissection

Transplants

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Microinjection

DNA Transgenics

• BAC
• Meganuclease
• Sperm-mediated

Ventricles

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Microscopy

Confocal

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Molecular Biology Protocols

Bacterial Transformations

Northern Blots

Northern Blots fro MicroRNA's

Plasmid Organization

• Stocks

Plasmid Preps

PCR

• General PCR set up for zebrafish
• Single cell PCR for zebrafish embryos

RT-PCR

• RT-PCR for zebrafish embryos
• RT-PCR from zebrafish explants
• RT-PCR for frogs

Restriction Digests

Southern Blots

Western Blots

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Mutagenesis

Mutagenesis Protocol from Stratagene

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Staining

H & E Staining

Histology for Frogs

Immunocytochemistry

• Antibodies
• Acetylated tubulin and RM044 immunostaining

In situ Hybridization

• In situ protocol for zebrafish embryos
• Frog in situ hybridization protocol

TUNEL