The Sive lab works on the function and regulation of a number of ectodermally expressed genes in Xenopus laevis and Danio rerio

(This page under construction)

• Xenopus HoxD1
  
  
  • HoxD1 is related to Drosophila labial and is a paralogue of HoxA1 and HoxB1.
    
    
  • HoxD1 information at the XMMR
    
    
  • Xenopus HoxD1 IS predominantly expressed in the ectoderm of early neurula embryos
    (transverse section of a stage 14 HoxD1 stained embryo, dorsal at the top).
    
    
  • HoxD1 is expressed in the dorsal ectoderm with an anterior limit in the posterior hindbrain.
    (double staining with HoxD1 and Krox20 in r3 and r5)
    
    
  • HoxD1 is expressed during gastrula and early neurula stages (time course of expression).
    
    
  • Xenopus HoxD1 (and HoxA1) References:
    • Kolm and Sive (1995) Dev Biol 167: 34-49
    • Kolm and Sive (1994). Biochem Soc Trans 22: 579-584
    • Sive and Cheng (1991). Genes Dev 5: 1321-32
    • Blumberg B, Wright CV, De Robertis EM, Cho KW (1991) Science 253: 194-196
      
      
  • Sequences:
    • HoxD1 (Xhox.lab1) nucleotide sequence
    • HoxD1 protein sequence
      
      
  • Hox links
    • The labial Homeobox Page
    • Extensive Drosophila labial information: structure, expression, regulation, and function
      (comparision to vertebrate labial-like Hox genes too)
    • Thomas Bürglin's Homeobox Page
    • Alex Spirov's Hox Database
      
      
  • Xenopus cement gland markers
  
  
  • XCG - expressed exclusively in the cement gland
    • XCG expression pattern from the Molecular Marker Database (see below for a comparison to XAG and XA)
      
      
  • XAG - expressed in the cement gland and hatching gland
    • XAG nucleotide sequence
    • XAG protein sequence
    • XAG expression pattern from the Molecular Marker Database (see below for a comparison to XCG and XA)
    • XAG plasmid map
      
      
  • XA - expressed in the posterior cement gland and hatching gland
    • XA nucleotide sequence
    • XA protein sequence
    • XA expression pattern from the Molecular Marker Database (see below for a comparison to XCG and XAG)
      
      
  • Comparison of XCG, XAG, and XA expression in neurula and tailbud embryos (courtesy of Leila Bradley)
    
    
  • Cement gland references:
    • A general review: Sive, H.L. and Bradley, L.C. Developmental Dynamics (1996) 205(3):265.
    • Bradley, L.C., Wainstock, D. and Sive, H.L. (1996) Development122: 2739-2750
    • A. Hemmati-Brivanlou, D. Frank, M. E. Bolce, B. D. Brown, H. L. Sive & R. M. Harland (1990) Development 110: 325-30
    • H. L. Sive, K. Hattori & H. Weintraub (1989) Cell 58: 171-80

• Xenopus otx2
  

  From Laura Gammill's Home Page: My research interests center on understanding the events involved in the anteroposterior patterning of the ectoderm in Xenopus, specifically how the expression of the anteriorly expressed homeobox gene Xotx2 is correctly localized and regulated in its ability to effect cement gland differentiation.

  • Otx2 sequences
    • Nucleotide: Pannese et al, Blitz and Cho
    • Protein: Pannese et al, Blitz and Cho
      
      
  • Otx2 references
    • Gammill, L. S. and Sive, H. (1997) Development.124:2471-2481
    • Pannese M, Polo C, Andreazzoli M, Vignali R, Kablar B, Barsacchi G, Boncinelli E (1995) Development 121 (3): 707-720
    • Blitz IL, Cho KW (1995). Development 121 (4): 993-1004
    • Kablar, B., Vignali, R., Menotti, L., Pannese, M., Andreazzoli, M., Polo, C., Giribaldi, M.G., Boncinelli, E. and Barsacchi, G. (1996) Mech. Dev. 55,145-158
      
      
  • Vertebrate otx information from the Boncinelli lab
    
    
  • Extensive Drosophila orthodenticle information: structure, expression, regulation, and function (comparision to the function of vertebrate orthodenticle-like (otx) genes too)
• many other genes

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