Transcriptome Comparison

Transcriptome: Comparison with published mRNA levels

Gene

Description

mRNA copies/cell

this study¹

Iyer & Struhl²

SAGE³

RPS2

Ribosomal protein S2

50.1
75
57.6

SPT15

TATA-binding protein

7.1
ND
1.2

RAS2

GTP-binding protein

3.5
ND
1.7

RPO21

RNAP II, largest subunit

2.9
ND
0.2

FAR1

Inhibitor of CDK-cyclin

2.6
ND
0.2

DED1

RNA helicase

2.6
25
0.2

TRP3

Anthranilate synthase

2.5
1.9
<0.2⁴

CDC28

CDK

2.2
ND
2.0

HAT1

Histone Ac transferase

0.9
ND
no tag⁵

POL12

DNA polymerase subunit

0.8
ND
no tag⁵

NUP157

Nuclear pore protein

0.8
ND
0.5

PRP4

Splicing factor

0.7
ND
0.3

PEX14

Peroxin

0.7
ND
0.2

RAD52

Repair, recombination

0.6
ND
no tag⁵

GAL1

Galactokinase

0.2
<0.1
no tag⁵

HIS3

IGP dehydratase

(1.1)⁶
7
no tag⁵

Gene	Description	mRNA copies/cell
this study¹	Iyer & Struhl²	SAGE³
RPS2	Ribosomal protein S2	50.1	75	57.6
SPT15	TATA-binding protein	7.1	ND	1.2
RAS2	GTP-binding protein	3.5	ND	1.7
RPO21	RNAP II, largest subunit	2.9	ND	0.2
FAR1	Inhibitor of CDK-cyclin	2.6	ND	0.2
DED1	RNA helicase	2.6	25	0.2
TRP3	Anthranilate synthase	2.5	1.9	<0.2⁴
CDC28	CDK	2.2	ND	2.0
HAT1	Histone Ac transferase	0.9	ND	no tag⁵
POL12	DNA polymerase subunit	0.8	ND	no tag⁵
NUP157	Nuclear pore protein	0.8	ND	0.5
PRP4	Splicing factor	0.7	ND	0.3
PEX14	Peroxin	0.7	ND	0.2
RAD52	Repair, recombination	0.6	ND	no tag⁵
GAL1	Galactokinase	0.2	<0.1	no tag⁵
HIS3	IGP dehydratase	(1.1)⁶	7	no tag⁵

¹based on HDA analysis and a total number of 15000 polyA RNA molecules per cell
²based on quantitative hybridization of oligo's to RNA (V. Iyer & K. Struhl, PNAS 93, 5208-5212, 1996)
³based on a total number of tags of 60633 and 15000 polyA RNA molecules per cell (V.E. Velculescu et al., Cell 88, 243-251, 1997)
⁴the tag was not unique to this mRNA
⁵no tag exists for this RNA
⁶HIS3 is not appropriate for comparison because the strain used was modified at the HIS3 locus