Reproducibility

To assess the reproducibility of the HDA technology, RNA was harvested from two independent wild type colonies and the two RNA preparations were hybridized to two different HDAs on two separate days. The results were plotted on a histogram in which the ratio of levels of each mRNA measured from the two independent experiments (fold change) was plotted against the number of genes whose mRNAs have been counted in that category. For 99% of all genes scored, the expression values produced for each gene in the two experiments were within 1.7-fold. The genes whose expression values scored outside this narrow range in the two experiments tended to be genes whose expression levels are near the limits of detection (0.1 mRNA molecules/cell). Thus, the technology generates highly reproducible results.