Summary of Results and Insights

• a revised description of the yeast mRNA population including transcripts expressed at very low levels
• the relative stability of yeast mRNAs
• genome-wide expression is equivalently dependent on Srb4 and Rpb1, suggesting the holoenzyme is generally recruited to promoters of protein-coding genes
• distinct genome-wide expression signatures are obtained when specific components of the transcription apparatus are inactivated, suggesting models for activator-factor interaction
• genome-wide expression results can predict and explain phenotypes
• putative targets of signal transduction pathways can be identified by comparing genome expression signatures from these experiments and those which modify the cellular environment
• chromatin modifying components can have both positive and negative roles in vivo
• databases describing genome-wide dependence on components of the transcriptional machinery are useful tools for testing and developing models of gene regulation
• Table of Results