Genetic Reagents
Three types of mutations have proven to be useful for determining which genes depend on the function of a component of the transcription apparatus. For essential components of the apparatus (e.g. Rpb1), temperature-sensitive mutations are valuable because they permit conditional growth of cells and they allow the investigator to examine effects on gene expression at any point after factor inactivation. For non-essential components with known enzymatic activities (e.g. Srb10), point mutations which knock out catalytic function are useful to discern the role of the enzymatic activity. For non-essential components with no apparent enzymatic activity (e.g. Srb5), complete deletion mutations have been used.
Project Goals
Technology, Protocols, and Analysis